Phytochemical screening, Toxicity and Antidiabetic Effect of Extract of Gotu Kola Leaves (Centella asiatica Urban)

Gotu gotu leaves (Centella Asiatica (L.) Urban) are wild plants that grow widely as grasses used by the community as traditional medicinal plants. This study aims to determine the antidiabetic potential of gotu gotu leaf extract originating from Indonesian, Toba Samosir Regency, North Sumatra Province. Results of the secondary metabolite phytochemical test contained in n-hexane, ethyl acetate and ethanol of gotu leaf extract. The results of phytochemical screening showed that n-hexane flavonoid extracts, saponins and steroids; ethyl acetate; saponins and steroids; Ethanol contains flavonoids, saponins, steroids, and tannins. The toxicity test used the BSLT (Brine Shrimp Lethality Test) method. The results of the toxicity test of Gotu Gotu leaf extract (Centella Asiatica (L.) Urban) against Artemia salina Leach larvae showed that the n-hexane extract LC50 was 199.53; ethyl acetate: 133.43 ethanol has toxic properties with an LC50 value of 110.03μg/ml. Antidiabetic in vitro with amylase enzyme inhibition test, as a comparison is acarbose obtained IC50 = 20.267 ppm; ethilasetate: 121.909 ppm and ethanol: 108.797 ppm.