Identification of <i>Toxoplasma gondii</i> antigenic proteins using an <i>in vivo</i> approach and <i>in silico</i> investigation of their polymorphism
<i>Toxoplasma gondii</i> is a unique species that exhibits genotype diversity related to clinical virulence. Currently, genotyping is restricted, which limits epidemiological knowledge of the strains. To overcome this limitation, we aimed to develop serotyping tests. First, we used a murine <i>in vivo</i>, non-targeted experimental approach based on proteomics techniques through which we were able to identify a panel of more than 700 antigenic proteins from <i>T. gondii</i>. Then, we analyzed the polymorphism of these proteins using a whole-genome sequencing database containing the genomes of 117 genotyped strains. We showed that none of the 986 non-silent SNPs detected is specific to the strain type. The <i>in vivo</i> approach is the first that allowed the identification of such a large panel of antigenic proteins. Moreover, the polymorphism analysis, the first based on a large next-generation sequencing database, showed the limits that currently restrict the development of a serotyping technique.
Authors
- Christelle Pomarès (ORCID: https://orcid.org/0000-0002-6192-3293)
- Laetitia Beal
- Coralie L’Ollivier (ORCID: https://orcid.org/0000-0002-1086-3745)
- Chloé Gommenginger
- Sandrine Houzé (ORCID: https://orcid.org/0000-0001-9016-3847)
- O. Villard
- Hervé Pelloux (ORCID: https://orcid.org/0000-0001-5727-5441)
- Isabelle Villena (ORCID: https://orcid.org/0000-0002-7415-4198)
- Anne-Sophie Deleplancque
- Bernard Cimon
- Hélène Fricker Hidalgo
- Julie Denis (ORCID: https://orcid.org/0009-0009-6294-9338)
- Alexander W. Pfaff (ORCID: https://orcid.org/0000-0003-3482-1803)
- Luc Paris (ORCID: https://orcid.org/0000-0003-4722-4469)
Institutions
- Centre National de la Recherche Scientifique (FR)
- Inserm (FR)
- Université Côte d'Azur (FR)
- Université Paris Cité (FR)
- Aix-Marseille Université (FR)
- Université de Lille (FR)
- Centre Hospitalier Universitaire de Grenoble (FR)
- Sorbonne Université (FR)
- Méditerranée Infection Foundation (FR)
- Assistance Publique Hôpitaux de Marseille (FR)
- Sorbonne Paris Cité (FR)
- Mère et enfant en milieu tropical : pathogènes, système de santé et transition épidémiologique (FR)
- Assistance Publique – Hôpitaux de Paris (FR)
- Centre Hospitalier Universitaire de Reims (FR)
- Laboratoire National de Référence (MA)
- Centre Hospitalier Universitaire d'Angers (FR)
- Pitié-Salpêtrière Hospital (FR)
- Lille Inflammation Research International Center (FR)
- Hôpital Bichat-Claude-Bernard (FR)
- Hôpitaux Universitaires de Strasbourg (FR)
- Institut d’Epidémiologie Neurologique et de Neurologie Tropicale (FR)
- Institut de Recherche pour le Développement (FR)
- Université d'Angers (FR)
- Université de Strasbourg (FR)
- Université Grenoble Alpes (FR)
- Université de Reims Champagne-Ardenne (FR)
Publication Details
- Journal
- Microbiology Spectrum
- Published
- 2025-03-26
- DOI
- https://doi.org/10.1128/spectrum.02040-24
- Primary Topic
- Toxoplasma gondii Research Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00