Deep Learning for Detection and Morphological Analysis of Stomatal Complexes in Microscopic Imaging

The accurate detection and classification of stomatal complexes play a critical role in understanding plant physiology, particularly in areas such as gas exchange regulation and water conservation. Leveraging advancements in computer vision and deep learning, this study introduces a robust framework for the automated identification and morphological analysis of stomata cells within microscopic images. A large annotated dataset was utilized to train and evaluate a deep learning model capable of localizing stomatal complexes with high precision. Employing advanced object detection architectures, our model achieved high performance metrics, demonstrating its efficacy in distinguishing stomatal regions from surrounding epidermal cells. The developed system not only facilitates rapid and accurate detection but also contributes to improved morphological analysis, allowing re-searchers to quantitatively assess stomatal features under various conditions. This approach promises significant contributions to plant sciences, providing a scalable tool for cellular analysis that may inform studies on plant adaptation, environmental stress response, and crop improvement. Our findings illustrate the potential of integrating computer vision with biological research, setting a precedent for future work in automated plant cell analysis.

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Publication Details

Published
2025-02-06
DOI
https://doi.org/10.1109/cictn64563.2025.10932631
Primary Topic
AI in cancer detection
Type
article
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article

Deep Learning for Detection and Morphological Analysis of Stomatal Complexes in Microscopic Imaging

Sanjeev Kumar Shukla, Vikas Shukla, Pawan Kumar, Kuldeep Pal
AI in cancer detection
article

Deep Learning for Detection and Morphological Analysis of Stomatal Complexes in Microscopic Imaging

Sanjeev Kumar Shukla, Vikas Shukla, Pawan Kumar, Kuldeep Pal
article en

Abstract

The accurate detection and classification of stomatal complexes play a critical role in understanding plant physiology, particularly in areas such as gas exchange regulation and water conservation. Leveraging advancements in computer vision and deep learning, this study introduces a robust framework for the automated identification and morphological analysis of stomata cells within microscopic images. A large annotated dataset was utilized to train and evaluate a deep learning model capable of localizing stomatal complexes with high precision. Employing advanced object detection architectures, our model achieved high performance metrics, demonstrating its efficacy in distinguishing stomatal regions from surrounding epidermal cells. The developed system not only facilitates rapid and accurate detection but also contributes to improved morphological analysis, allowing re-searchers to quantitatively assess stomatal features under various conditions. This approach promises significant contributions to plant sciences, providing a scalable tool for cellular analysis that may inform studies on plant adaptation, environmental stress response, and crop improvement. Our findings illustrate the potential of integrating computer vision with biological research, setting a precedent for future work in automated plant cell analysis.

Indian Institute of Technology Kanpur (IN)
Openalex Percentile: Top 97%
AI in cancer detection
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Deep Learning for Detection and Morphological Analysis of Stomatal Complexes in Microscopic Imaging — Sanjeev Kumar Shukla, Vikas Shukla, et al. · (2025) | TGRS Research Map | TGRS