Non-muscle myosin II inhibition at the site of axon injury increases axon regeneration
Motor axon regeneration following peripheral nerve injury is critical for motor recovery but therapeutic interventions enhancing this are not available. We conduct a phenotypic screen on human motor neurons and identified blebbistatin, a non-muscle myosin II inhibitor, as the most effective neurite outgrowth promotor. Despite its efficacy in vitro, its poor bioavailability limits in vivo application. We, therefore, utilize a blebbistatin analog, NMIIi2, to explore its therapeutic potential for promoting axon regeneration. Local NMIIi2 application directly to injured axons enhances regeneration in human motor neurons. Furthermore, following a sciatic nerve crush injury in male mice, local NMIIi2 administration to the axonal injury site facilitates motor neuron regeneration, muscle reinnervation, and functional recovery. NMIIi2 also promotes axon regeneration in sensory, cortical, and retinal ganglion neurons. These findings highlight the therapeutic potential of topical NMII inhibition for treating axon damage.
Authors
- Bruna Lenfers Turnes (ORCID: https://orcid.org/0000-0002-3989-8394)
- Selwyn S. Jayakar (ORCID: https://orcid.org/0000-0002-5792-6408)
- Laura Perrault
- J. Tabitha Hees (ORCID: https://orcid.org/0000-0001-6022-6907)
- Clifford J. Woolf (ORCID: https://orcid.org/0000-0002-6636-3897)
- Xirui Chen (ORCID: https://orcid.org/0000-0002-7767-2716)
- Xiangsunze Zeng
- Georgios Kimourtzis (ORCID: https://orcid.org/0009-0005-4049-1769)
- Michael Condro (ORCID: https://orcid.org/0000-0001-9591-4814)
- Keunjung Heo
- Harley I. Kornblum (ORCID: https://orcid.org/0000-0002-3779-4540)
- Elisa Fazzari (ORCID: https://orcid.org/0000-0001-5651-8587)
- Zhigang He (ORCID: https://orcid.org/0000-0001-6080-6880)
- Aparna Bhaduri (ORCID: https://orcid.org/0000-0003-4625-6899)
- Jed L. Hubbs (ORCID: https://orcid.org/0000-0002-3923-3392)
- Kuchuan Chen
- Zhuqiu Xu (ORCID: https://orcid.org/0000-0002-6259-2668)
- Maryam Arab (ORCID: https://orcid.org/0000-0002-9575-0511)
- Tammy Szu‐Yu Ho
- Xuan Song
- Roshan Pandey
- Lee Barrett
- Kathleen Zhang
Institutions
- Boston Children's Hospital (US)
- Harvard University (US)
- University of California, Los Angeles (US)
Publication Details
- Journal
- Nature Communications
- Published
- 2025-03-26
- DOI
- https://doi.org/10.1038/s41467-025-58303-6
- Citations
- 7
- Primary Topic
- Nerve injury and regeneration
- Type
- article
- Field-Weighted Citation Impact
- 6.36
Funders
- Spinal Muscular Atrophy Foundation
- Dr. Miriam and Sheldon G. Adelson Medical Research Foundation
- National Institutes of Health
- University of California, Los Angeles
- National Institute of Neurological Disorders and Stroke