Connectome-driven neural inventory of a complete visual system

Vision provides animals with detailed information about their surroundings and conveys diverse features such as colour, form and movement across the visual scene. Computing these parallel spatial features requires a large and diverse network of neurons. Consequently, from flies to humans, visual regions in the brain constitute half its volume. These visual regions often have marked structure-function relationships, with neurons organized along spatial maps and with shapes that directly relate to their roles in visual processing. More than a century of anatomical studies have catalogued in detail cell types in fly visual systems<sup>1-3</sup>, and parallel behavioural and physiological experiments have examined the visual capabilities of flies. To unravel the diversity of a complex visual system, careful mapping of the neural architecture matched to tools for targeted exploration of this circuitry is essential. Here we present a connectome of the right optic lobe from a male Drosophila melanogaster acquired using focused ion beam milling and scanning electron microscopy. We established a comprehensive inventory of the visual neurons and developed a computational framework to quantify their anatomy. Together, these data establish a basis for interpreting how the shapes of visual neurons relate to spatial vision. By integrating this analysis with connectivity information, neurotransmitter identity and expert curation, we classified the approximately 53,000 neurons into 732 types. These types are systematically described and about half are newly named. Finally, we share an extensive collection of split-GAL4 lines matched to our neuron-type catalogue. Overall, this comprehensive set of tools and data unlocks new possibilities for systematic investigations of vision in Drosophila and provides a foundation for a deeper understanding of sensory processing.

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Journal
Nature
Published
2025-03-26
DOI
https://doi.org/10.1038/s41586-025-08746-0
Citations
46
Primary Topic
Neurobiology and Insect Physiology Research
Type
article
Field-Weighted Citation Impact
41.82
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article

Connectome-driven neural inventory of a complete visual system

Sanna Koskela, Brandon S Canino, Sandro Romani, Wei Qiu et al.
46 citations
Nature
Neurobiology and Insect Physiology Research
41.82
article

Connectome-driven neural inventory of a complete visual system

Sanna Koskela, Brandon S Canino, Sandro Romani, Wei Qiu, Kit D. Longden, Laura E. Burnett, Louis K. Scheffer, Gary Patrick Hopkins, John J Walsh, William T. Katz, Edward M. Rogers, C. Shan Xu, Pavithraa Seenivasan, Tansy Yang, Stuart Berg, Donald J. Olbris, Samantha Finley-May, Zhiyuan Lu, Stephan Saalfeld, Michael Cook, Emily A Yakal, Ashley L Scott, Alexander Thomson, Philipp Schlegel, Eric T. Trautman, Jan Funke, Aljoscha Nern, Stephan Preibisch, Miriam A Flynn, Christopher Ordish, Reed George, Eyal Gruntman, John Bogovic, Philip M. Hubbard, Judith Hoeller, Imran Hameed, Patricia K. Rivlin, Lowell Umayam, Alanna Lohff, Wyatt Korff, Michael B. Reiser, Arthur Zhao, Gregory S.X.E. Jefferis, Shin-ya Takemura, Marisa Dreher, Tyler Paterson, Christopher Knecht, Michał Januszewski, Alexandra M. C. Fragniere, Frank Loesche, Harald F. Hess, Kenneth J. Hayworth, Charli Maldonado, Stephen M. Plaza, Nneoma Okeoma, Louis Anthony Scuderi, Shirley A Lauchie, Caroline Mooney, Satoko Takemura, Emily M Phillips, Tobias Pietzsch, Julie Kovalyak, Iris Talebi, Jody Clements, Ting Zhao, Nathan C Klapoetke, Gerald M. Rubin, Gary B. Huang, Meghan Leonard, Jennifer Rivas Salinas, Claire Walsh
article en
46 citations

Abstract

Vision provides animals with detailed information about their surroundings and conveys diverse features such as colour, form and movement across the visual scene. Computing these parallel spatial features requires a large and diverse network of neurons. Consequently, from flies to humans, visual regions in the brain constitute half its volume. These visual regions often have marked structure-function relationships, with neurons organized along spatial maps and with shapes that directly relate to their roles in visual processing. More than a century of anatomical studies have catalogued in detail cell types in fly visual systems<sup>1-3</sup>, and parallel behavioural and physiological experiments have examined the visual capabilities of flies. To unravel the diversity of a complex visual system, careful mapping of the neural architecture matched to tools for targeted exploration of this circuitry is essential. Here we present a connectome of the right optic lobe from a male Drosophila melanogaster acquired using focused ion beam milling and scanning electron microscopy. We established a comprehensive inventory of the visual neurons and developed a computational framework to quantify their anatomy. Together, these data establish a basis for interpreting how the shapes of visual neurons relate to spatial vision. By integrating this analysis with connectivity information, neurotransmitter identity and expert curation, we classified the approximately 53,000 neurons into 732 types. These types are systematically described and about half are newly named. Finally, we share an extensive collection of split-GAL4 lines matched to our neuron-type catalogue. Overall, this comprehensive set of tools and data unlocks new possibilities for systematic investigations of vision in Drosophila and provides a foundation for a deeper understanding of sensory processing.

NatureVol. 641(8065)
Howard Hughes Medical Institute (US), MRC Laboratory of Molecular Biology (GB), University of Toronto (CA), Janelia Research Campus (US), University of Cambridge (GB), Scarborough Health Network (CA), Google (Switzerland) (CH)
Openalex Percentile: Top 0%
Neurobiology and Insect Physiology Research
41.82
DOIOpen Access PDFOpenAlex
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