Chromosome-scale genome assembly of Trigonella corniculata (L.)L. (Nagauri pan /Kasuri methi), an important spice

Trigonella corniculata (L) L. or Nagauri pan /Kasuri methi, is an important spice crop with high nutraceutical potential. We report the de novo chromosome-scale assembly of T. corniculata genome using high coverage PacBio, Illumina and Hi-C reads. The assembly spans 798 Mb (Megabases) in 282 scaffolds with a scaffold N50 of 99.6 Mb. More than 98% of the sequence length is captured in eight different pseudomolecules with an average length of 98 Mb. A BUSCO score of over 97% is suggestive of the high degree of completeness and contiguity of the genome. A total of 64,801 protein-coding genes are predicted. Genome-wide Simple Sequence Repeats (99,149) have been identified and wet lab validated at forty-eight loci. The chromosome-scale genome assembly of T. corniculata and the SSR markers identified in this study will provide a strong foundation for future structural and functional genomics studies in T. corniculata and other fenugreek species.