Systematic Investigation of Tumor Microenvironment and Antitumor Immunity With IOBR

ABSTRACT The tumor microenvironment (TME) is a critical factor in antitumor immunity and treatment outcome in cancer therapy. We have developed an analysis tool called the immuno‐oncology biological research (IOBR) to investigate the TME and its role in antitumor immunity. Leveraging multi‐omics data, IOBR facilitates comprehensive analysis of TME characteristics, immune interactions, and their impact on immunotherapy outcomes. IOBR features six modules for TME analysis, including transcriptomic data preprocessing, TME profiling, TME pattern identification, ligand–receptor interaction analysis, genome‐TME interaction assessment, and visualization, along with modeling. Since its release, this tool has been widely applied in many studies. In the future, IOBR will gradually integrate TCR/BCR repertoire analysis, enhance genomic functionalities, and develop spatial transcriptomics modules, which will further our understanding of TME dynamics and tumor immunity.