OPTIMIZATION OF COVALENT DNA IMMOBILIZATION FOR CONTACT PRINTING METHOD

The contact printing method is widely used to create microchips and develop highly sensitive biosensors. This method is based on techniques for immobilizing biomolecules. In this work, two effective methods for covalent immobilization of oligonucleotides, based on the reactions of disulfi de bond formation and azide-alkyne cycloaddition, were optimized for the contact printing method. With both immobilization strategies, oligonucleotides retain the ability to hybridize with complementary DNA, which provides the basis for their practical use in bioanalytical applications.