Molecular Characterization of Virulent Genes in Neonatal Respiratory Infections Associated Pathogenic Strains of Pseudomonas aeruginosa Using PCR Technique

The current research study anticipated the identification of toxic genes in Pseudomonas aeruginosa involved in quorum sensing (QS), as many virulence factors are regulated by QS. The objective of the current research was to isolate and screen the pathogenic strains of P. aeruginosa from neonate’s sputum samples and molecular characterization of virulent genes in these strains. The P. aeruginosa was screened out in the Microbiology Laboratory, Department of Zoology, Government College University Lahore. Different identification tests and Molecular characterization was conducted. Molecular characterization of virulent genes was performed by using specific primers. Products of PCR were sequenced in order to get accession numbers from the NCBI site. The identification of genes LasR, LasI, rhlR, and rhlI was made in the P. aeruginosa strains (SS5, SS6, and SS11). Recent studies have disclosed that these virulent genes play a significant role in P. aeruginosa strains’ resistance against various antibacterial agents. P. aeruginosa possesses diverse metabolic capabilities to manage survival in various conditions and can exhibit extreme resistance to antibiotics, facilitating its spread among different environments especially in hospitals. These genes involved in overall pathogenicity of P. aeruginosa.