Multiplex one-step RT–qPCR assays for simultaneous detection of BRV, BCoV, Escherichia coli K99+ and Cryptosporidium parvum
The one-step multiplex RT-qPCR method developed in this study for detecting BRV, BCoV, <i>E. coli</i> K99<sup>+</sup>, and <i>C. parvum</i> is expected to be an effective tool for the rapid and economical diagnosis and monitoring of diarrhoeal diseases in calves.
Authors
- Yingying Yang (ORCID: https://orcid.org/0000-0002-3859-5244)
- Huatao Li (ORCID: https://orcid.org/0000-0003-0252-9541)
- Dehua Yin
- Xinru Zhao
- Haihui Gao
- Zhi Cao (ORCID: https://orcid.org/0000-0002-8189-2121)
- Kaiqiang Fu (ORCID: https://orcid.org/0000-0001-5502-7292)
- Yidan Wang (ORCID: https://orcid.org/0000-0002-9108-1285)
- Min Li (ORCID: https://orcid.org/0000-0002-0188-1394)
- Xiaoxuan Zheng (ORCID: https://orcid.org/0009-0006-5986-0365)
Institutions
- Qingdao Agricultural University (CN)
- Sinovac Biotech (CN)
- Ningxia Academy of Agriculture and Forestry Sciences (CN)
Publication Details
- Journal
- Frontiers in Veterinary Science
- Published
- 2025-03-26
- DOI
- https://doi.org/10.3389/fvets.2025.1561533
- Citations
- 4
- Primary Topic
- Viral gastroenteritis research and epidemiology
- Type
- article
- Field-Weighted Citation Impact
- 5.58