Enhancing D-lactic acid production from methane through metabolic engineering of Methylomonas sp. DH-1
The metabolic engineering strategies employed in this study, including the use of an inducible promoter and alleviation of ADP-glucose accumulation toxicity, successfully enhanced the ability of the strain to produce D-LA from methane. Furthermore, optimizing the bioreactor fermentation process through methane and nitrate supplementation resulted in a significant increase in both the titer and productivity, exceeding previously reported values.
Authors
- Wooyoung Park (ORCID: https://orcid.org/0009-0004-8397-1124)
- Gwon Woo Park (ORCID: https://orcid.org/0000-0001-6362-4046)
- Seungwoo Cha (ORCID: https://orcid.org/0000-0003-0236-9330)
- Myounghoon Moon (ORCID: https://orcid.org/0009-0005-8197-3617)
- Jae-Hwan Jo
- Ji-Sook Hahn
- Minsik Kim (ORCID: https://orcid.org/0000-0003-1095-840X)
- Jong Kwan Lee
Institutions
- Chonnam National University (KR)
- Seoul National University (KR)
- Korea Institute of Energy Research (KR)
Publication Details
- Journal
- Microbial Cell Factories
- Published
- 2025-03-25
- DOI
- https://doi.org/10.1186/s12934-025-02695-z
- Citations
- 2
- Primary Topic
- Microbial metabolism and enzyme function
- Type
- article
- Field-Weighted Citation Impact
- 1.61
Funders
- National Research Foundation of Korea