Comparative analysis of metagenomic next-generation sequencing for pathogenic identification in clinical body fluid samples
Whole-cell DNA mNGS demonstrates significantly higher sensitivity for pathogen detection and identification compared to both cfDNA mNGS and 16S rRNA NGS in clinical body fluid samples, particularly those associated with abdominal infections. However, the compromised specificity of wcDNA mNGS highlights the necessity for careful interpretation in clinical practice.
Authors
- Ning Sun (ORCID: https://orcid.org/0000-0002-9585-2374)
- Wentao Guo (ORCID: https://orcid.org/0000-0001-8058-8323)
- Deyu Gao
- Xinyi Xia (ORCID: https://orcid.org/0000-0002-8644-8599)
- Jiaxun Zhang (ORCID: https://orcid.org/0009-0002-3234-2569)
- Yong Chen (ORCID: https://orcid.org/0000-0003-4314-690X)
- Cao Jin (ORCID: https://orcid.org/0000-0001-6275-8069)
Institutions
- Guangdong Medical College (CN)
- Nanjing General Hospital of Nanjing Military Command (CN)
- Anhui Provincial Hospital (CN)
- Second Affiliated Hospital of Nanjing Medical University (CN)
- Nanjing University (CN)
Publication Details
- Journal
- BMC Microbiology
- Published
- 2025-03-25
- DOI
- https://doi.org/10.1186/s12866-025-03887-8
- Citations
- 7
- Primary Topic
- Gut microbiota and health
- Type
- article
- Field-Weighted Citation Impact
- 5.64
Funders
- National Natural Science Foundation of China