Generation of nanobodies against the F protein of respiratory syncytial virus and establishment of an indirect immunofluorescence assay
A respiratory syncytial virus (RSV) detection nanobody, F-E2, was successfully screened by constructing a dromedary camel immune library. F-E2 binds to RSV F with ng affinities and can be used for Western blot, enzyme-linked immunosorbent assay, flow cytometry, immunohistochemistry, and immunofluorescence assays. Importantly, a high-throughput RSV immunofluorescence detection method based on F-E2 has been established and proved to be highly specific, sensitive, cost-effective, and fast, with great application potential.
Authors
- Zekai Cheng
- Sandra Chiu (ORCID: https://orcid.org/0000-0001-9034-5755)
- Entao Li (ORCID: https://orcid.org/0000-0003-0666-8557)
- Jiachen Zhang (ORCID: https://orcid.org/0000-0002-6488-6686)
- Qianqian Wang
- Dongxiang Hong
- Tengchuan Jin (ORCID: https://orcid.org/0000-0002-1395-188X)
Institutions
- University of Science and Technology of China (CN)
- Anhui Provincial Center for Disease Control and Prevention (CN)
Publication Details
- Journal
- Microbiology Spectrum
- Published
- 2025-03-25
- DOI
- https://doi.org/10.1128/spectrum.02784-24
- Primary Topic
- Respiratory viral infections research
- Type
- article
- Field-Weighted Citation Impact
- 0.00