Genetic- and culture-based tools for studying <i>Bacteroides fragilis</i>

The relatively limited availability of genetic tools has hampered mechanistic studies of <i>Bacteroides fragilis,</i> an opportunistic anaerobe that constitutes 1%-5% of the gut microbiota in healthy humans. Here we describe novel vectors for <i>B. fragilis</i> gene deletion and expression as well as a semi-defined media for cultivation of <i>B. fragilis</i> str. P207.

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Publication Details

Journal
Microbiology Resource Announcements
Published
2025-03-25
DOI
https://doi.org/10.1128/mra.00006-25
Citations
1
Primary Topic
Bacterial Identification and Susceptibility Testing
Type
article
Field-Weighted Citation Impact
1.32

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article

Genetic- and culture-based tools for studying Bacteroides fragilis

Aretha Fiebig, Sean Crosson, Matthew K. Schnizlein, Abhishek Anil Dubey
1 citations
Microbiology Resource Announcements
Bacterial Identification and Susceptibility Testing
1.32
article

Genetic- and culture-based tools for studying Bacteroides fragilis

Aretha Fiebig, Sean Crosson, Matthew K. Schnizlein, Abhishek Anil Dubey
article en
1 citations

Abstract

The relatively limited availability of genetic tools has hampered mechanistic studies of <i>Bacteroides fragilis,</i> an opportunistic anaerobe that constitutes 1%-5% of the gut microbiota in healthy humans. Here we describe novel vectors for <i>B. fragilis</i> gene deletion and expression as well as a semi-defined media for cultivation of <i>B. fragilis</i> str. P207.

Microbiology Resource AnnouncementsVol. 14(5)
Leibniz Institute on Aging - Fritz Lipmann Institute (FLI) (DE), Michigan State University (US)
National Institute of General Medical Sciences, National Institute of Diabetes and Digestive and Kidney Diseases
Zero hunger
Openalex Percentile: Top 21%
Bacterial Identification and Susceptibility Testing
1.32
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