ANALYSIS OF THE COMPOSITION OF BIOLOGICALLY ACTIVE SUBSTANCES AND DEVELOPMENT OF QUALITY INDICATORS OF HYDRO ALCOHOLIC EXTRACT FROM THE PEEL OF POMEGRANATE FRUIT (PUNICA GRANATUM L.)

Taking into account the WHO data characterizing the increase in the resistance of periodontopathogenic microflora of the oral cavity to a significant number of antibiotics, the use of herbal medicines in the complex therapy of periodontal diseases, which often have a fairly high antimicrobial activity, seems promising. According to the available literature, the possibility of obtaining an extraction agent from the waste of the processing of pomegranate fruits, which is characterized by a wide range of pharmacological activity due to the presence of a polyphenol complex, seems interesting. The purpose of this work is to analyze the composition of polyphenolic substances contained in the extract from raw materials obtained from the waste of pomegranate fruit processing and to assess the total content of gallic acid in the raw materials. For identification and quantitative determination of phenolcarboxylic acids, fresh pomegranate fruits of the Guleisha rozovaya, Kizil-anor varieties and a varietal mixture were used that meet the quality indicators regulated by GOST 27573-87 "Fresh pomegranate fruits", which were subjected to grinding and mechanical juice pressing, after which they were dried in an isothermal mode at 100 C and ground to a particle size passing through a sieve with a hole diameter of 2 mm. At the preliminary stage of the study, the authors assessed the waste output during the processing of pomegranate fruits to obtain juice products and showed the feasibility of using the waste to obtain aqueous-alcoholic extracts. Chromatographic analysis confirmed the identity of the substances contained in the extracts obtained with 50, 70 and 90% ethyl alcohol. Gallic, ellagic acids, catechin and epigallocatechin gallate were identified in all samples. The quantitative content of gallic acid in fresh pomegranate pulp was 1.2%. The relative error of the method for determining the content of gallic acid with a 95% probability is ±2.3% for fresh raw materials.