ADDITIVELY MANUFACTURED MICROFLUIDICS FOR SEQUENTIAL FLOW CONTROL IN AMPLIFICATION ASSAYS

The adoption of colorimetric readout in detecting diseases at the point of care is increasing due to its simple analysis, easy interpretation, and compatibility with highly specific nucleic acid amplification assays like Loop-mediated amplification (LAMP).However, combining colorimetric readout with amplification assays presents challenges such as extensive user involvement in the sequential assay steps (~7 steps), interuser variability in assay handling, and quantification of colorimetric signals, limiting its application at point of need [1].Implementation of microfluidic systems for amplification assays (with sequential steps) often requires complex, extensive microfabrication techniques, and user involvement to an extent [2].To overcome these challenges, we introduce a deployable Stereolithography (SLA) leveraged microfluidic system for automated amplification-on-chip via sequential flow control in amplification assays (Figure 1).