HIGH-THROUGHPUT TITRATION VIA SEGMENTED FLOW IN A LAB-ON-A-CHIP DEVICE FOR ENVIRONMENTAL SENSING APPLICATIONS

We introduce a high-throughput titration technique within a lab-on-a-chip (LOC) system, reducing sample usage and assay time, and demonstrate its application for pH determination necessary in total alkalinity (TA) measurements.LOC devices, including an autonomous opto-fluidic analyzer developed by our group [1], enabled conducting in-situ titration-based assays, crucial for water quality monitoring through measuring various parameters, e.g., TA and dissolved inorganic carbon (DIC) [2,3].In LOC-based titration, each point usually involves filling the entire device with a sample and titrant, maintaining a single dilution factor, = + , and