A DISPOSABLE, PASSIVE MICROFLUIDIC DEVICE FOR POINT-OF-CARE DETECTION OF SARS-COV-2 ANTIBODY USING HEMAGGLUTINATION ASSAY

We introduce a disposable microfluidic chip that utilizes an integrated passive pumping technique for efficient SARS-CoV-2 antibody (AB) detection in user blood, requiring minimal input.This device passively pumps and mixes the blood sample with the reagent, enabling rapid AB detection within three minutes.The hemagglutination test (HAT) involves the clumping of red blood cells (RBCs) in the presence of antibodies specific to a virus (Figure 1) [1].A couple of studies have applied the hemagglutination technique to develop card-based, point-of-care (PoC) platforms for self-testing of 3].Although these tests are simple and easy to interpret, the need for manual operation introduces user variability.To address this, we developed a device that uses a pressure-driven pumping mechanism using centrifuge tubes to achieve passive mixing of the blood sample and the reagent, thereby eliminating user intervention.