ELECTROCHEMICAL-BASED POINT-OF-CARE DETECTION OF G6PD THROUGH BI-SUBSTRATE REACTION

Objectives: Glucose-6-phosphate dehydrogenase (G6PD) is a vital enzyme present in red blood cells that protects them against oxidative damage by generating the reduced form of nicotinamide adenine dinucleotide phosphate [1], by catalyzing a redox reaction between glucose-6-phosphate (G6P) and NADPH, forming 6-phospho-D-glucono-1,5-lactone and NADP.G6PD deficiency results in several disorders including neonatal hyperbilirubinemia, and acute and chronic hemolysis; prevalent in African, Asian, and Mediterranean regions.Genetic variations in this enzyme result in reduced stability and activity, leading to an elevated susceptibility to cellular hemolysis during periods of oxidative stress.Traditionally, spectrophotometric enzyme activity tests have been employed to identify G6PD deficiency [2-4].However, these assays are inaccurate and require sophisticated pathological labs.Hence, a need for a point-of-care biosensor that is capable of measuring the G6PD levels by utilizing G6P and NADPH.In this work, we designed an electrochemical-based sensor to identify and quantify the levels of G6PD on a modified carbon electrode printed paper-based device.