Catalytic hairpin assembly-coupled CRISPR/Cas12a biosensor for sensitive detection of melamine in dairy products
We combined catalytic hairpin assembly (CHA) with the Cas12a system for detecting melamine adulteration. This system involved two-step signal conversion and two-level amplification, boosting the sensor's versatility and sensitivity. The sensor showed excellent specificity and applicability for melamine detection in dairy products, and was broadened to viral nucleic acid detection.
Authors
- Bang‐Ce Ye (ORCID: https://orcid.org/0000-0002-5555-5359)
- Huadong Li (ORCID: https://orcid.org/0000-0001-7085-9775)
- Bin‐Cheng Yin (ORCID: https://orcid.org/0000-0002-4011-4307)
- Pei‐Qiang Ma (ORCID: https://orcid.org/0000-0002-4046-0795)
- Yue Liu (ORCID: https://orcid.org/0000-0002-0092-7485)
- Huijuan Cheng
Institutions
- East China University of Science and Technology (CN)
- Chinese Academy of Sciences (CN)
- Shanghai Advanced Research Institute (CN)
- Zhejiang University of Technology (CN)
Publication Details
- Journal
- Chemical Communications
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d5cc00114e
- Citations
- 3
- Primary Topic
- Advanced Nanomaterials in Catalysis
- Type
- article
- Field-Weighted Citation Impact
- 1.55