Some Factors Affecting Free and Immobilized Amylase Activity

In our study, we prepared chitosan beads and immobilized bacterial amylase enzyme on these beads; Parameters such as comparing the optimization conditions (pH, pH stability, temperature, thermal stability, substrate concentration) of free bacterial amylase and amylase immobilized on chitosan beads and investigating the effect of immobilization in its usage areas are included. The immobilized enzyme showed better activity at both lower and higher pH levels compared to the free enzyme. When considering pH stability, the results were consistent with the pH activity values. In other words, both free and immobilized amylase exhibited the highest activity at the same pH levels. However, the free amylase showed less stability at low and high pH levels compared to the immobilized amylase. In terms of temperature-dependent activity, it was found that both free and immobilized enzymes showed the best activity at the same temperature. However, the immobilized enzyme showed activity at higher temperatures than the free enzyme. Moreover, when studying the effect of substrate concentration on free and immobilized enzymes, both types of enzymes exhibited the best activity in the same concentration of starch solution. In this study, factors affecting immobilized and free amylase activity were examined.