A Qualitative Analysis of Caffeine from Non-Alcoholic Beverages Using UV-Visible Spectrophotometer

Abstract: Caffeine is a widely consumed psychoactive compound found in various non-alcoholic beverages, including tea, coffee, and energy drinks. This study focuses on the qualitative analysis of caffeine using UV-Visible spectrophotometry, a rapid and reliable technique for detecting caffeine content. Samples were prepared using liquid-liquid separation method and analysed at their characteristic absorption wavelength, ensuring precise detection. The UV-Visible spectrophotometer, operating within a range of 200 nm to 400 nm, is sensitive to caffeine detection, providing precision and cost-effectiveness. Out of the 30 samples tested, 13 contained caffeine, despite all being labelled as non-alcoholic beverages without any indication of caffeine content. The research not only focuses on the toxicological analysis of caffeine but also highlights the importance of accurate labelling regarding its presence. The findings have significant implications for consumer awareness, regulatory bodies, and manufacturers, underscoring the need for accurate labelling of caffeine content.