ISSR analysis for the evaluation of shrub polymorphism in the genus Corylus L.

In this study the genetic polymorphism of Corylus avellana and C. pontica cultivars in the collections of the Federal Scientific Center of Agroecology of the Russian Academy of Sciences, Volgograd region, Russia (cadastre No. 34:36:000014:177; No. 34:34:000000:122) was evaluated using ISSR-primers. The results of two methods of DNA isolation from young leaves are presented: 1. D-Plants kit for DNA isolation from plants, Biolabmix according to the protocol; 2. Modified method with cetyltrimethylammonium bromide (CTAB) with 0.2% β-mercaptoethanol. A large proportion of primers (74%) showed no differences in DNA methylation levels, which can be explained by their low specificity. Only 6 (UBC807, UBC811, UBC826, UBC835, UBC841, UBC890) of the 23 primers showed clear and interpretable bands. They may be useful for breeding work to separate hazelnut genotypes and cultivars. Primer UBC811 showed the maximum number (11) of loci in the spectrum, of which 8 were polymorphic, with loci lengths ranging from 250-1230 base pair. The polymorphism level ranged from 33.3 to 72.7%. Of the recommended 6 primers, the UBC807 primer ranked second in terms of the number of polymorphic loci, with UBC890 ranking last. Comparative evaluation of the results concerning the number of bands on primers UBC811 and UBC826 in scientific works of other researchers showed that they were generally comparable. The detected polymorphism (16.67%) between C. pontica cultivars confirms their genetic relationship.