Molecular Insights into the Positive Role of Soybean Nodulation by GmWRKY17

Soybean is an important economic oilseed crop, being rich in protein and plant oil, it is widely cultivated around the world. Soybeans have been shown to establish a symbiotic nitrogen fixation (SNF) with their compatible rhizobia, resulting in the formation of nodules. Previous studies have demonstrated the critical roles of phytohormones, such as abscisic acid and cytokinin, in the process of legume nodulation. The present study investigated the role of GmWRKY17, a homolog of <i>Rosa hybrida</i> (<i>Rh</i>)<i>WRKY13</i> in regulating plant immunity through cytokinin content and abscisic acid signaling in soybean nodulation. Utilizing real-time PCR and histochemical staining, we demonstrated that <i>GmWRKY17</i> is predominantly expressed in soybean root nodules. Subsequently, we analyzed the function of <i>GmWRKY17</i>-overexpression, RNA interference (RNAi), and the CRISPR/Cas9 system. Overexpression of <i>GmWRKY17</i> significantly increases soybean nodule number, while RNAi or CRISPR/Cas9-mediated knockout of <i>GmWRKY17</i> resulted in a dramatic repression of nodule formation in soybeans. These results highlight that GmWRKY17 functions as a positive regulator involved in soybean nodulation. Furthermore, manipulation of <i>GmWRKY17</i> expression impacts the expression of genes associated with the nod factor (NF) signaling pathway, thereby influencing soybean nodulation. This study demonstrated that WRKY-type transcription factors are involved in the regulation of legume nodulation, offering new light on the molecular basis of the symbiotic interaction between legumes and rhizobia.