The LAMP-CRISPR-Cas13a technique for detecting the CBASS mechanism of phage resistance in bacteria
The study findings highlight the regulatory role of the QS network in APD systems. Notably, this is the first study to develop an innovative biotechnological application for the LAMP-CRISPR-Cas13a rapid-technique (<2 h), thereby helping to optimize phage therapy by detecting bacterial resistance mechanisms and predicting the potential inefficacy of therapeutic phages and thus improving patient prognosis.
Authors
- Inés Bleriot (ORCID: https://orcid.org/0000-0002-1846-4693)
- Belén Aracil (ORCID: https://orcid.org/0000-0002-6156-8252)
- Antonio Barrio-Pujante (ORCID: https://orcid.org/0009-0006-8173-0823)
- María Tomás (ORCID: https://orcid.org/0000-0003-4501-0387)
- Laura Fernández-García (ORCID: https://orcid.org/0000-0002-8531-6105)
- Concha Ortiz-Cartagena (ORCID: https://orcid.org/0000-0001-6632-1454)
- Daniel Pablo‐Marcos (ORCID: https://orcid.org/0000-0003-1716-0329)
- Lucía Blasco (ORCID: https://orcid.org/0000-0002-4039-4142)
- Lucía Armán (ORCID: https://orcid.org/0009-0008-6209-327X)
- Clara Ibarguren-Quiles (ORCID: https://orcid.org/0009-0004-3909-7257)
- Jorge Calvo
- Felipe Fernández-Cuenca (ORCID: https://orcid.org/0000-0002-6597-6130)
- Patricia Fernández-Grela
Institutions
- Universidade da Coruña (ES)
- Instituto de Salud Carlos III (ES)
- Hospital Universitario Virgen Macarena (ES)
- Marqués de Valdecilla University Hospital (ES)
- Centro Nacional de Microbiologia (ES)
- Instituto de Investigación Marqués de Valdecilla (ES)
Publication Details
- Journal
- Frontiers in Microbiology
- Published
- 2025-03-24
- DOI
- https://doi.org/10.3389/fmicb.2025.1550534
- Citations
- 5
- Primary Topic
- Bacteriophages and microbial interactions
- Type
- article
- Field-Weighted Citation Impact
- 8.58