Absolute abundance calculation enhances the significance of microbiome data in antibiotic treatment studies
Flow cytometry is a laborious method, but identified a higher number of significant microbiome changes in comparison to common compositional data analysis and even revealed to be superior to a spike-in method. Calculation of absolute abundances and GCN correction are valuable methods that should be standards in microbiome analyses in veterinary as well as human medicine.
Authors
- Klaus Neuhaus (ORCID: https://orcid.org/0000-0002-6020-2814)
- Thilo M. Fuchs (ORCID: https://orcid.org/0000-0002-3056-1824)
- Lena-Sophie Paul
- Angelika Grümpel-Schlüter (ORCID: https://orcid.org/0009-0000-5408-8392)
- Jeannette Kluess (ORCID: https://orcid.org/0000-0001-6877-6508)
- Stefanie Wagner (ORCID: https://orcid.org/0000-0002-3873-7011)
- Michael Weber (ORCID: https://orcid.org/0000-0001-5397-4085)
Institutions
- Friedrich-Loeffler-Institut (DE)
- Leibniz-Institute for Food Systems Biology at the Technical University of Munich (DE)
Publication Details
- Journal
- Frontiers in Microbiology
- Published
- 2025-03-24
- DOI
- https://doi.org/10.3389/fmicb.2025.1481197
- Citations
- 12
- Primary Topic
- Gut microbiota and health
- Type
- article
- Field-Weighted Citation Impact
- 9.67