Label-free metabolic fingerprinting of motile mammalian spermatozoa with subcellular resolution
Our approach eliminates the need for sperm immobilization and labeling and captures the native state of sperm metabolism. This technique could be adapted for metabolism-based sperm selection for assisted reproduction.
Authors
- Dalibor Pánek (ORCID: https://orcid.org/0000-0002-1177-3848)
- Fitore Kusari (ORCID: https://orcid.org/0000-0001-6977-810X)
- Zdeněk Trachtulec (ORCID: https://orcid.org/0000-0001-5772-9394)
- Aleš Benda (ORCID: https://orcid.org/0000-0001-5829-8362)
- Lenka Backová
Institutions
- University of the Basque Country (ES)
- Czech Academy of Sciences (CZ)
- Charles University (CZ)
- Max Planck Institute for the Study of Religious and Ethnic Diversity (DE)
- Instituto Biofisika (ES)
- Fundación Biofísica Bizkaia (ES)
- Institute of Molecular Genetics (RU)
- Czech Academy of Sciences, Institute of Molecular Genetics (CZ)
Publication Details
- Journal
- BMC Biology
- Published
- 2025-03-24
- DOI
- https://doi.org/10.1186/s12915-025-02167-1
- Primary Topic
- Sperm and Testicular Function
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Ministerstvo Školství, Mládeže a Tělovýchovy
- Grantová Agentura České Republiky
- Akademie Věd České Republiky
- European Regional Development Fund