Metabolic modeling of fatty liver disease – a factor for the progradient course of polycystic ovary syndrome

Metabolic dysfunction-associated fatty liver disease (MAFLD) is a multifactorial liver disease that often develops in patients with polycystic ovary syndrome (PCOS), is accompanied by hormonal imbalance, chronic inflammation and may be combined with iron overload. This article presents a simulation modeling of MAFLD with multiple organ dysfunction syndrome (MODS) caused by combined intake of excess saturated fat (palm oil), carbohydrates (fructose) and inorganic iron (ferrous sulfate) with histological confirmation of changes in the liver and other organs. The approach to the correction of such MODS was tested using a complex agent containing 1200 mg of inositols in two forms in the ratio of 5:1 – myoinositol 1000 mg and D-chiro-inositol 200 mg, combined with folic acid (200 mg) and manganese pyroglutamate (pydolate) (5 mg) in 1 sachet (a biologically active food supplement «Dikirogen»). Analysis of the complex pattern of changes in biochemical parameters and improvement in histological parameters of liver, kidney, heart and brain slices due to the administration of pharma-nutraceutical indicates the efficacy of its use on the modeling of MAFLD. The normalization of elevated levels of ferritin, transferrin saturation coefficient and serum iron levels was observed in experimental animals with pharmaco-nutraceutical «Dikirogen», as well as regression of signs of MODS achieved during the experiment, which was manifested by (1) normalization of elevated levels of white blood cells, (2) restoration of creatinine and glomerular filtration rate (GFR) levels (nephroprotection), (3) restoration of aminotransferase and total protein levels (hepatoprotection). Positive histological confirmations of the above described biochemical findings were obtained. Thus, the experimental model shows that Dikirogen can contribute to the regression of MODS associated with the development of MAFLD in iron overload. Key words: metabolic dysfunction-associated fatty liver disease, polycystic ovary syndrome, myoinositol, D-chiroinositol, ferroptosis, hemosiderosis, palm oil, fructose, animals