Marivivens marinum sp. nov., isolated from tidal flat, Gochang, South Korea

A Gram-negative, rod-shaped, non-motile, aerobic bacterium, designated MK-3^T, was isolated from shallow seawater in Gochang, Republic of Korea. Growth of strain MK-3^T occurred at 15-40 °C (30 °C), pH 6.0-7.0 (pH 7.0) and in the presence of 2-3 % NaCl (2%). Phylogenetic analysis based on the 16S rRNA gene sequence placed strain MK-3^T within the family <i>Roseobacteraceae</i>. It exhibited sequence similarities of 95.7% with <i>Salipiger marinus</i> CK-I3-6^T, <i>Salipiger aestuarii</i> DSM 22011^T and <i>Salipiger pentaromativorans</i> P9^T; 95.4% with <i>Ruegeria alba</i> 1NDH52C^T, <i>Salipiger manganoxidans</i> VSW210^T and <i>Salipiger thiooxidans</i> DSM 10146^T; 95.3% with <i>Marivivens donghaensis</i> AM-4^T and <i>Histidinibacterium lentulum</i> B17^T; and 95.2% with <i>Marivivens geojensis</i> FJ12^T. Phylogenetic and phylogenomic analyses consistently demonstrated that strain MK-3^T formed a distinct lineage within the genus <i>Marivivens</i>, clustering with its closest relatives. The major fatty acids were C_{18 : 1} <i> ω7</i>c<i>/</i>C1_{8 : 1} <i> ω6</i>c, C_{18 : 1} <i> ω7</i>c 11-methyl and C_{16 : 0}. The genome length of strain MK-3^T was 3.3 Mbp, and the DNA G+C content was 62.8 mol%. The strain contained Q-10 as the major ubiquinone. The polar lipids consisted of three phosphatidylinositol mannosides and a diphosphatidylglycerol. Based on its phenotypic, chemotaxonomic, phylogenetic and genomic characteristics, strain MK-3^T represents a novel species in the genus <i>Marivivens</i>, for which the name <i>Marivivens marinum</i> sp. nov. is proposed. The type strain is MK-3^T (=KEMB 21417^T=KCTC 8294^T=JCM 36630^T).