Exploring The Therapeutic Potential of Xanthoparmelia pulla (Ach.) O. Blanco, A. Crespo, Elix, D. Hawksw. & Lumbsch (Parmeliaceae)

Aim of study: This study aims to investigate the antioxidant effect and potential toxicity of the methanol extract of X. pulla species in Turkey and to identify the secondary metabolites of this species. Area of study: Samples were collected from Yozgat Bozok University East Campus. Material and method: Detection of secondary metabolites in X. pulla was performed using HPLC. Antioxidant activity was evaluated using DPPH● and ABTS●+ methods. Cytotoxicity was evaluated using MTT assay in MCF-7 and MDA-MB-231 cell lines. Main results: The highest secondary metabolite was salazinic acid (37.23±9.21 mg/extract). Total phenol content was 172.77±10.50 mg GAE/g and flavonoid content was 9.25±1.32 mg CA/g. The extract showed 87.41% DPPH● radical scavenging activity at 2 mg/mL concentration and effectively neutralized ABTS●+ radical at all concentrations. In terms of cytotoxicity, MCF-7 cell viability was inhibited by 66.84% at 125 μg/mL and MDA-MB-231 cell viability was inhibited by 39.42% at 250 μg/mL. Research highlights: This study reveals the biological activity of X. pulla and its potential for natural product based drug development.