Techniques for Validating CRISPR Changes Using RNA-Sequencing Data
The inadvertent modifications identified by the evaluation of 4 CRISPR experiments highlight the value of using RNA-seq to identify transcriptional changes to cells altered by CRISPR, many of which cannot be recognized by evaluating DNA alone. Specific guidelines are presented for designing and analyzing CRISPR experiments using RNA-seq data.
Authors
- David A. Largaespada (ORCID: https://orcid.org/0000-0002-3183-0491)
- Susan K. Rathe (ORCID: https://orcid.org/0000-0002-2340-5217)
- Branden S. Moriarity (ORCID: https://orcid.org/0000-0002-3031-3767)
- Elizabeth N. Edwards
- Kyle Williams (ORCID: https://orcid.org/0000-0003-4333-9111)
- James E. Johnson (ORCID: https://orcid.org/0000-0002-2590-4765)
- Tracy A. Marko
- Jyotika Varshney (ORCID: https://orcid.org/0000-0003-0275-1521)
- Paige Hazelton Ridder
Institutions
- University of Minnesota (US)
- University of Minnesota Medical Center (US)
Publication Details
- Journal
- Genes
- Published
- 2025-03-24
- DOI
- https://doi.org/10.3390/genes16040369
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Children's Tumor Foundation