Sequential single-enzyme oxidation of 5-hydroxymethylfurfural to 2,5-furandicarboxylic acid by an engineered lanthanide-dependent alcohol dehydrogenase
A lanthanide-dependent alcohol dehydrogenase, PedH, was identified, which possessed the rare ability to continuously oxidize HMF to produce FDCA. A fully automated workflow was developed to modify PedH, resulting in an increased yield of FDCA.
Authors
- Lun Wang
- Haoran Yu (ORCID: https://orcid.org/0000-0001-9012-4688)
- Lirong Yang (ORCID: https://orcid.org/0000-0002-6378-8451)
- Ling Jiang (ORCID: https://orcid.org/0000-0002-0391-6941)
- Ke Liu (ORCID: https://orcid.org/0000-0001-9698-817X)
- Qunfeng Zhang (ORCID: https://orcid.org/0000-0002-2301-1816)
- Jianping Wu (ORCID: https://orcid.org/0000-0002-9924-7307)
Institutions
- Zhejiang University of Science and Technology (CN)
- Zhejiang University (CN)
Publication Details
- Journal
- Green Chemistry
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d5gc00157a
- Citations
- 7
- Primary Topic
- Catalysis for Biomass Conversion
- Type
- article
- Field-Weighted Citation Impact
- 4.72
Funders
- National Natural Science Foundation of China