Influencia de la concentración de infusiones de hojas de Quercus sideroxyla en la extracción de compuestos fenólicos, actividad antioxidante y antiinflamatoria

Quercus sideroxyla infusion has been shown to have important biotherapeutic effects, especially antioxidant and anti-inflammatory effects due to its content of polyphenolic compounds. Among the main variables for the extraction of bioactive compounds by infusing in water stands out the solute-solvent ratio. The purpose of this study was to analyze the influence of the percentage of leaves on the phenolic content extracted and its antioxidant and anti-inflammatory potential effects. To this end, infusions of Quercus 1 at 10% (w/v) of leaves were obtained, which were analyzed for the content of phenols and total flavonoids, phenolic profiling for phenolic acids, flavonoids and hydrolyzable tannins was monitored by means of UPLC-ESI--MS/MS. Antioxidant activity was determined using FRAP, ABTS, ORAC, and DPPH, as well as anti-inflammatory activity using an erythrocyte membrane stabilization assay. The variation in the percentage of leaves to produce the infusions promoted a progressive increase in the extraction of phenols to a percentage of 7% (w/v), subsequently no significant differences were observed, but a nominal decrease in the content was. The phenolic profiling was also affected, favoring a higher concentration of oak leaves and the extraction of hydrolyzable tannins, especially vascalagin and penducalagin 1 and 2, which contributed to increasing the antioxidant activity for the trapping of peroxide radicals and reduction of the ferric ion. Anti-inflammatory activity was also increased, although it appears to be more associated with a synergistic effect between epicatechin gallate and other polyphenols.