COMPARATIVE ANALYSIS OF ERYTHROCYTES PLASMA MEMBRANE OBTAINED BY ATOMIC FORCE MICROSCOPY IN PATIENTS WITH MILD AND SEVERE PANCREATITIS

Background. Atomic force microscopy in the diagnosis of various diseases is being used more and more every year. Purpose. To analyze the state of the erythrocyte membrane in patients with mild and severe pancreatitis. Materials and methods. Two groups of patients were included in the multicenter study. All patients underwent atomic force microscopy of erythrocytes obtained from peripheral venous blood using an atomic force microscope. Blood from donors was examined as a control group. Statistical processing was performed using the Statistica 6.1 program. The linear dimensions, area and volume of red blood cells and the shape and condition of the erythrocyte membrane were compared during the study. Results. When visualizing the plasma membrane in patients with mild acute pancreatitis, the appearance of blebbing was noted. In severe acute pancreatitis, erosions of the erythrocyte membrane were detected. Conclusion. The use of atomic force microscopy as a test makes it possible to notice a tendency to disease progression in the early stages in the absence of other changes. The use of atomic force microscopy makes it possible to quickly detect changes in the severity of the patient's condition even before the appearance of clinical and laboratory changes. Monitoring of the state of red blood cells during atomic force microscopy is necessary for timely correction of the treatment.