Isolation and FISH mapping of three repetitive DNAs in <i>Capsicum annuum</i> L.

In this study, three tandem repeats were identified from the genome of the Capsicum annuum L. C26 and C37 were satellite repeats, and C28 was a minisatellite repeat. Monomers of C26, C37, and C28 were 152 bp, 37 bp, and 180 bp respectively. Fluorescence in situ hybridization (FISH) results revealed that signals of C26 were located at the centromeric region of a pair of chromosomes and the subtelomeric region of a pair of chromosomes; signals of C28 were located on eight pairs of chromosomes; signals of C37 were mainly located at the subterminal region of all chromosomes, but some signals at one end of the chromosome and some at both ends of the chromosome. Furthermore, double-color FISH results showed that seven pairs of chromosomes could be reliably distinguished by specific FISH signals of C28+C37. It will help to construct molecular karyotypes, reveal new chromosome rearrangements, and improve chromosome engineering breeding of the Capsicum genus.