Development of a strain-specific PCR as a diagnostic tool for surveillance, detection, and monitoring of vancomycin-resistant Enterococcus faecium during outbreak
These PCR tests maintain the accuracy and discriminatory power of WGS for identifying the ST117-CT469 outbreak strain but are more cost-effective, faster, and easier to use compared to WGS. They enhance VREfm outbreak management by providing an efficient method for rapid screening. Application of strain-specific PCR based on WGS data is currently the most effective screening method during large, ongoing outbreaks.
Authors
- Martine De Vos (ORCID: https://orcid.org/0000-0002-0496-7038)
- Erik Bathoorn (ORCID: https://orcid.org/0000-0003-2319-8147)
- Lilli Gard (ORCID: https://orcid.org/0000-0001-9182-8986)
- Mariëtte Lokate (ORCID: https://orcid.org/0000-0001-8664-3557)
- Monika Fliss
- Viktoria Akkerboom
- Artur J. Sabat
- Robin F. J. Benus
Institutions
- University Medical Center Groningen (NL)
- University of Groningen (NL)
- Stichting Certe Medische Diagnostiek en Advies (NL)
Publication Details
- Journal
- Antimicrobial Resistance and Infection Control
- Published
- 2025-03-24
- DOI
- https://doi.org/10.1186/s13756-025-01538-1
- Citations
- 2
- Primary Topic
- Antimicrobial Resistance in Staphylococcus
- Type
- article
- Field-Weighted Citation Impact
- 2.79