The effect of the pesticide Klaxon on the oxidative stress and the antioxidant responses of the Zebra mussel (Dreissena polymorpha)

Klaxon pesticide represents a novel generation of insecticide employed in the control of diseases, pests and weeds in select agricultural regions. Pesticides that enter the aquatic environment indirectly have a detrimental impact on the organisms that inhabit this environment, and humans are ultimately exposed to these chemicals through the food chain. The present study investigated the toxicity of the klaxon pesticide in Dreissena polymorpha, a suitable bioindicator of water pollution, through the analysis of oxidative stress and metabolic biomarkers. The effects of klaxon at concentrations of 0.01, 0.15 and 0.30 mg·L-1 on oxidative stress and antioxidant changes in D. polymorpha were determined over a 24 – and 96–hour period. Enzyme–linked immunosorbent assay (ELISA) kits were employed to quantify the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and thiobarbituric acid (TBARS), as well as to assess the levels of reduced glutathione (GSH). ELISA kits were used to determine results. Statistical evaluation of biomarker analyzes was performed using the SPSS 24.0 package program one–way ANOVA (Duncan 0.05) test. Significant decreases in GSH levels (P<0.05); significant increases in TBARS levels (P<0.05) were observed. Significant decreases (P<0.05) in SOD, CAT and GPx activities were observed. Considering the study data, it was determined that the klaxon pesticide penetrating the body of the living organism caused oxidative stress and changes in enzyme activities in D. polymorpha.