Clinico-pathological studies on granulosa cell tumor in a Shitzu dog

A six years old Shih Tzu breed intact female dog was presented to department of veterinary clinical complex, with history of abdominal distension, exercise intolerance and normal food intake. On ultra sound examination, intestines appeared floating due to fibrinous fluid in abdomen and pleural effusions were also noticed upon x-ray examination. Cytology of ascitic fluid revealed activated mesothelial cells with clusters of cells showing anaplasia. Exploratory laparotomy was conducted upon which bilateral ovarian masses appearing soft, nodular and hyperaemic within ovarian bursa were retrieved. Impression smears showed ‘Call-Exner’ bodies pattern. Histopathological examination revealed neoplastic polyhedral to fusiform epithelial cells with abundant vacuolated eosinophilic cytoplasm, clustered in solid and acinar pattern supported by varying amount of fibrous connective tissue. Immunohistochemistry showed strong positivity for inhibin-α. The case was confirmed as granulosa cell tumor based on gross, cytological, histopathological and immunohistochemical examinations.