Role of Adiponectin in Regulating Cytokines and Its Contribution to the Occurrence and Progression of Clinical Mastitis in Holstein Cows

Cytokines are crucial in various physiological and pathological processes, especially in inflammatory diseases in mammals. However, the comprehensive identification of cytokines and their potential regulatory functions in the mammary glands of Holstein cows suffering from clinical mastitis (CM) remains only partially understood. This study aimed to systematically identify biological processes (BPs) and differentially expressed proteins (DEPs) associated with cytokines and to explore their functions through the analysis of previously obtained data from data-independent acquisition (DIA) proteomics. We confirmed that the dynamic balance between pro- and anti-inflammatory factors is closely associated with dairy mastitis. A total of 4 BPs, comprising 75 upregulated and 16 downregulated DEPs, were identified, particularly in relation to adiponectin (ADIPOQ), which strongly interacts with the other DEPs and participates in peroxisome proliferator-activated receptor (PPAR) and adipocytokine signaling pathways. Immunohistochemical and immunofluorescence staining revealed that ADIPOQ was predominantly localized in the cytoplasm of mammary epithelial cells. Moreover, the expression levels of <i>ADIPOQ</i> mRNA and protein in the mammary glands of the CM group were notably reduced compared to those in the healthy group. A potential mechanism of action of ADIPOQ was suggested, with findings indicating that a decrease in ADIPOQ expression could potentially worsen inflammation in CM. These results offer novel insights into cytokines and the regulatory mechanisms of ADIPOQ in Holstein cows with CM which may benefit the prevention and treatment of dairy mastitis.