Evaluation of the potential roles of kisspeptin and neurokinin genes in puberty treatment

Objective: Kisspeptin (KP) and neurokinin (NK) are neuropeptides that govern the reproductive endocrine axis through regulating hypothalamic neuronal activity and pulsatile.Their critical role in reproductive health was first described by the demonstration of congenital hypogonadotropic hypogonadism and pubertal developmental anomalies following loss of function due to mutations in genes encoding the KP or NK signaling.This study aimed to investigate the efficacy of KP and NK, in mesenchymal stem cell (MSC)-based treatment of different origin, and examine the relationship of the expression of these genes with the passage number.Methods: Three adult rats (Rattus norvegicus) were used in our study.MSCs were obtained from bone marrow and adipose tissue of rats.The MSCs were analysed by flow cytometry using surface markers and RNA (ribonucleic acid) isolation was performed.Gene expressions of KP, NK and their receptors were investigated by real-time Makale Dili "Trke"/