A rapid and efficient in vivo inoculation method for introducing tree stem canker pathogens onto leaves: suitable for large-scale assessment of resistance in poplar breeding progeny
Compared to the conventional "in vitro stem segment inoculation method", the leaf method has the advantages of abundant inoculation materials, easy operation, rapid disease onset, and almost no adverse effect on the host. It is particularly suitable for the resistance screening of all progeny and the early (seedling) phenotypic selection of resistant poplar clones in poplar stem disease resistance breeding. The "in vivo leaf inoculation method" holds significant promise in poplar breeding, tree pathology, and molecular biology research on tree stem diseases.
Authors
- Huixiang Liu (ORCID: https://orcid.org/0000-0003-3953-0335)
- Yuchen Fu (ORCID: https://orcid.org/0000-0002-7743-3635)
- Jinxia Feng
- Xiaohua Su (ORCID: https://orcid.org/0000-0001-8427-4727)
- Wanna Shen (ORCID: https://orcid.org/0009-0008-4968-960X)
- Yinan Zhang (ORCID: https://orcid.org/0000-0002-0362-1473)
- Zheng Li
- Bingyu Zhang
- Long Pan (ORCID: https://orcid.org/0000-0002-8992-5899)
- Jiaping Zhao (ORCID: https://orcid.org/0000-0002-2054-6989)
- Yutian Suo
Institutions
- Chinese Academy of Forestry (CN)
- Research Institute of Forestry (CN)
- Shandong Agricultural University (CN)
Publication Details
- Journal
- Plant Methods
- Published
- 2025-03-24
- DOI
- https://doi.org/10.1186/s13007-025-01360-1
- Primary Topic
- Plant Pathogens and Fungal Diseases
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China