Comparison of automated systems using liquid microdilution for the determination of colistin resistance in Klebsiella pneumoniae strains

Objective: In the detection of colistin resistance in Klebsiella pneumoniae strains with multidrug resistance, automated systems give more erroneous results than the gold standard method, the liquid microdilution method.Comparative studies on the correct detection of colistin resistance by automated systems are limited in the literature.In this study; it is aimed to compare Vitek2 Compact and Phoenix automated systems by using liquid microdilution method in the determination of colistin susceptibility in K. pneumoniae strains isolated from various clinical specimens over a three-year period.Methods: Identification of K. pneumoniae strains was performed using conventional microbiological methods and automated bacterial identification systems which are Vitek2 (bioMrieux, MarcyL'Etoile, France) and Phoenix (Becton dickinson, M50 , USA).Antibiotic susceptibility tests were also performed with the same devices.Colistin sensitivity was determined Makale Dili "Trke"/