Selection of MDM2-binding peptides in <i>Escherichia coli</i> using an engineered split intein and aminoglycoside phosphotransferase
A protein <i>trans</i>-splicing (PTS)-based method for screening the peptides capable of binding to a target protein was developed using an engineered split intein and an aminoglycoside phosphotransferase. Two peptides selected against MDM2 bind to MDM2 with high affinity and a peptide can induce apoptosis in SJSA-1 cells.
Authors
- Kazuyuki Nobusawa
- Tsuyoshi Takahashi (ORCID: https://orcid.org/0000-0003-4949-9124)
- Yutaro Watanabe
- Chiori Tezuka
Institutions
- Gunma University (JP)
- Kiryu University (JP)
Publication Details
- Journal
- Chemical Communications
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d4cc06138a
- Primary Topic
- RNA and protein synthesis mechanisms
- Type
- article
- Field-Weighted Citation Impact
- 0.00