Creation of a heterotopic PDX model of uveal melanoma

The aim of the work is to create a subcutaneous PDX model of uveal melanoma, which will be further used to create a uveal melanoma metastasis. In the course of the work, a collection of 3 heterotopic PDX models of uveal melanoma was created, which, upon histological examination, showed that the PDX models of uveal melanoma correspond to the donor tumors. According to the results of our work, the rates of successful engraftment of the first generation tumor were 37.5% (3/8). An assessment of the growth dynamics of the obtained PDX models was carried out as well. According to the results, the growth rate of the first generation xenografts obtained from patients was quite low. Doubling of the tumor volume occurred in 42 days.To maintain the growth of the PDX model, tumors obtained from first generation mice were sequentially transplanted to the next group of mice. Our data show that the first generation models successfully engrafted in second generation mice. Moreover, the growth rate of the tumor node was considered to be higher than in the first generation.