Phlorizin Prolongs the Lifespan of <i>Caenorhabditis elegans</i> by Insulin and SIR-2.1 Regulation

Phlorizin has significant antioxidant properties and was studied using <i>Caenorhabditis elegans</i> to explore potential antiaging mechanisms. Results showed that phlorizin mitigated the harmful effects of high temperatures and hydrogen peroxide, reduced oxidative stress, increased antioxidant enzyme activity, and reduced malondialdehyde levels. Network pharmacological analysis reveals that the AKT1, INSR, and SOD2 signaling pathways play a key role in the antiaging effects of phlorizin. Its action is mediated by insulin and SIR-2.1, influencing DAF-16, SKN-1, and downstream genes in the antiaging effects. This implicates phlorizin as a promising functional food additive targeting the DAF-16 and SOD-3 axes for antiaging.