MiR-7-5p May Inhibit AML Cell Proliferation Via SKP2, KLF4, OGT Target Genes

Objective: Acute Myeloid Leukemia (AML) is distinguished by the differentiation and overgrowth of blast cells. In the current study, we purposed to elucidate the effect of miR-7-5p on AML cellular processes and the expression level of potential target genes. Methods: miR-7-5p mimic was transfected into AML cells by lipofectamine-mediated method and verified by qRT-PCR. The miR-7-5p's effect on proliferation and apoptosis was investigated by WST-8 and Caspase-3 kit (respectively). miRDB, miRTarBase, Targetscan, miRWalk, https://ongene.bioinfo-minzhao.org/, and http://soft.bioinfo-minzhao.org/lgl/a databases were utilized for in silico identification of possible target genes of miR-7-5p. Relative gene expression of potential target genes was investigated via the qRT-PCR technique. Results: In the group that is transfected with miR-7-5p, proliferation significantly decreased and apoptosis increased as against the control group. BCL2, SKP2, OGT, KLF4 and EGFR gene expression levels, which were determined as a result of possible target gene analysis by in silico methods and literature search, were investigated in AML cell lines. While the SKP2, KLF4 and OGT expression levels were statistically decreased in the group of transfected with mimic miR-7-5p, no statistically significant change was detected in the expressions of BCL2 and EGFR genes. Conclusion: miR-7-5p may affect the cancer process in AML by targeting SKP2, KLF4, and OGT genes. It is very important to identify and validate the miR-7-5p target genes, which has the possibility to be a new biomarker in the early diagnosis and therapy of AML. Therefore, our data obtained at mRNA level should be confirmed by further studies.