Layer‐by‐Layer Assembly of Plant Viruses Utilizing Specific Binding

Nanostructures can be fabricated from a new perspective using the bottom‐up method as an alternative to the top‐down approach. The previous investigations demonstrate the feasibility of utilizing plant viruses as building blocks for this purpose. Particularly promising is to take advantage of the specific interaction between Strep‐Tag II side chains on a modified tomato bushy stunt virus (TBSV) capsid and Strep‐Tactin. Herein, layer‐by‐layer structures consisting of Strep‐Tactin (first layer), Strep‐Tag II TBSV (second layer), Strep‐Tactin (third layer), and Strep‐Tag II TBSV (fourth layer) are reported on. Scanning force microscopy results confirm the successful assembly of this layer‐by‐layer viral assembly through specific interactions between each of the layers. This viral architecture offers significant potential for integration with other biological materials, paving the way for the development of practical biological nanodevices.