POTENTIAL APPLICATIONS OF Lactobacillus CELL COMPONENTS IN THE BIOTECHNOLOGY OF HUMAN LYMPHOBLASTOID INTERFERON

Aim. The study aimed to investigate the role of lactobacillus cell components and their effective use in increasing the efficiency of human lymphoblastoid interferon production. Methods. The study was conducted on the Namalva cell line, a known producer of human lymphoblastoid IFN-α. Newcastle disease virus (NDV) was used as an inducer of interferon production Lactobacillus cell components and metabolites cultured in MRS and its iodine- and Tween-modified variants were evaluated as co-inductors. Macromolecular platinum complexes were used to synchronize Namalva cells in the G1 phase, with synchronization assessed using flow cytometry. Results. Lactobacillus metabolites grown in MRSjt medium substantially increased interferon production when combined with NDV in synchronized Namalva cultures. The interferon yield increased 16-fold relative to the control. Synchronization of Namalva cells in the G1 phase using macromolecular platinum complex resulted in 96% of cells in the G1 phase, substantially boosting interferon production. Conclusions. Synchronizing Namalva cells in the G1 phase significantly improved IFN-α production when induced with NDV. The macromolecular platinum complex effectively synchronizes Namalva cells, optimizing interferon production. Lactobacillus metabolites from MRSjt medium serve as cost-effective, natural co-inductors for biotechnological IFN- α production.