Detection of staphylococcal enterotoxins A and B in cow milk using antigen capture enzyme-linked immunosorbent assay and dot-blot assays

Both AC-ELISA and dot-blot assays demonstrated considerable potential for detecting SEA and SEB in raw cow milk. The dot-blot assay exhibited superior sensitivity, whereas AC-ELISA offered higher specificity. These immunoassays provide viable alternatives to PCR, particularly in resource-limited settings, offering cost-effective and rapid detection of <i>S. aureus</i> enterotoxins. Further refinement and validation with larger sample sizes are necessary to enhance diagnostic accuracy and minimize cross-reactivity.