Electrochemical Effects of Encapsulating and Releasing New Synthesized Drug for Colorectal Cancer Therapy

ABSTRACT Cancer is one of the leading causes of cancer‐related deaths worldwide and is also among the most common malignant tumors of the digestive system. With the decreasing age of onset, there is an urgent need to develop effective therapeutic strategies to improve patient survival. RAN‐binding protein 9 (RANBP9) has been identified as an oncogene involved in several cancers, including colon cancer, gastric cancer, and lung cancer. It plays a crucial role in inhibiting tumor metastasis and enhancing chemotherapy sensitivity. In this study, we synthesized a novel two‐dimensional layered coordination polymer, CP1, by reacting Co(NO 3 ) 2 ·6H 2 O, HL, and NH(CN) 2 under solvothermal conditions at 120°C. The compound crystallizes in the monoclinic C2/m space group, with its asymmetric unit containing two Co(II) ions, two half N(CN) 2 − ligands, and one HL ligand. The Co(II) ions coordinate with N(CN) 2 − ligands to form a one‐dimensional chain, which is further interconnected by HL ligands to form a two‐dimensional layered structure. These layers interdigitate through van der Waals interactions, resulting in a three‐dimensional supramolecular framework (CP1). To enhance drug delivery and therapeutic effects, compound I was encapsulated within the conductive polymer PEDOP (poly(3,4‐ethylenedioxythiophene)) along with CP1. PEDOP, a well‐known conductive polymer, is highly conductive and stable, making it an ideal candidate for drug delivery systems. The resulting PEDOP‐CP1@I nanocarrier significantly inhibited the cellular activity of HT29 colorectal cancer (CRC) cells, upregulated RANBP9 expression, and modulated the expression of apoptosis‐related genes Bax and Bcl‐2, thereby inducing apoptosis in CRC cells. These findings highlight the potential of PEDOP‐CP1@I as a promising strategy for cancer therapy.